Express Gold Conjugation Kit

Express Gold Conjugation Kit

• Stable protein conjugation to 40 nm Gold
• Avidity binding retains protein functionality
• Simplify lateral flow assay development
Prototype Kits now available for evaluation

Description

Overview

IMRA’s Express Gold Conjugation kit enables stable protein conjugation to polymer-coated 40 nm gold nanoparticles (Activated Gold) by avidity binding. The kit features IMRA’s i-colloid gold and AnteoTech’s AnteoBind polymer and is provided with a simple protocol that does not require pH screening.

Features and Advantages

Quick – less than half a day to produce gold conjugates
Convenient – no pH screening required
Stable and gentle avidity binding – retain biomolecule functionality
Co-conjugate two proteins simultaneously
Reproducible antibody conjugation for consistent lateral flow results

Resources

Express Gold Conjugation Kit Instructions for Use

Prototype Kits available for Evaluation
ACAU40-2 (Mini) – contains 2 mL Gold*
ACAU40-10 (Midi) – contains 10 mL Gold*
ACAU40-50 (Maxi) – available soon
* All kits contain OD2 Activated Gold and Buffers
User supplies antibody/protein and blocking molecule – no pH optimization needed.

40 nm Gold Nanoparticles

Size Optimized for Lateral Flow

Product Features:

No capping agents or surfactants
High-purity laser-fabrication
Bare surface for efficient conjugation
No capping agents or surfactants
High colloidal stability during conjugation
Long shelf-life
Red color
Size optimized for lateral flow immunoassays
Use with 40 nm gold-platinum nanoparticles for multiplex lateral flow

Specifications:

Average Size (by disc centrifugation): 40 ± 3 nm
Size Distribution (%CV): <18%
Peak Wavelength: 524.0 ± 2.5 nm

Protocols:

Passive Conjugation of Proteins to i-colloid 40 nm OD1 Gold and Gold-Platinum Nanoparticles
Passive Conjugation of Proteins to i-colloid 40 nm OD5 and OD10 Gold Nanoparticles
i-colloid 40 nm Gold and Gold-Platinum Conjugation Optimization
Sequential Conjugation to i-colloid 40 nm Gold Nanoparticles (coming soon)

Technical Notes:

Consistent Lateral Flow Performance with i-colloid 40 nm Gold
Equivalent Performance of i-colloid 40 nm Gold and Gold-Platinum
Multicolor, Multiplex Lateral Flow with i-colloid Nanoparticles

20 nm Gold Nanoparticles

Laser-fabricated, Surfactant-free

The 20 nm Gold nanoparticles are fabricated by pulsed-laser ablation.

Product Features:

No capping agents or surfactants
High biomolecule conjugation
High purity and colloidal stability

Specifications:

Average Size (by disc centrifugation): 20 ± 2 nm
Size Distribution (%CV): <25%
Peak Wavelength: 521.0 ± 2.5 nm

Protocols:

Passive Conjugation of Proteins to i-colloid 20 nm Gold
I-colloid Gold and Gold-Platinum Conjugation Optimization
Sequential Conjugation to i-colloid 20 nm Gold

Technical Notes:

Imaging with Multi-functionalized i-colloid 20 nm Gold

40 nm Gold-Platinum Nanoparticles

Laser-fabricated, surfactant-free

The 40 nm Gold-platinum nanoparticles are fabricated by pulse-laser ablation.

Product Features:

High biomolecule conjugation with no capping agents or surfactants
Homogeneous Gold and Platinum distribution
Black color from absorption across entire visible spectrum
High purity and colloidal stability
Size optimized for lateral flow immunoassays
Use with 40 nm gold nanoparticles for multiplex lateral flow

Specifications:

Average Size (by disc centrifugation): 40 ± 5 nm
Gold-Platinum absorbs in entire visible spectrum: OD is measured at 400 nm

Protocols:

Passive Conjugation of Proteins to i-colloid 40 nm Gold and Gold-Platinum
i-colloid Gold and Gold-Platinum Conjugation Optimization

Technical Notes:

Equivalent Performance of i-colloid 40 nm Gold and Gold-Platinum
Multicolor, Multiplex Lateral Flow with i-colloid Nanoparticles