Lateral Flow Immunoassay

i-colloid 40 nm gold nanoparticles produced by IMRA’s pulsed-laser ablation process are stabilized without surfactants or capping agents such as citrate, and conjugates for lateral flow immunoassays can be generated by high-efficiency passive adsorption of antibodies to particle surfaces. The tightly controlled laser-ablation process generates gold colloids that produce consistent lateral flow assay results. Pulsed-laser ablation also enables production of gold-alloy nanoparticles, such as gold-platinum, that can be passively conjugated like gold but have optical signatures distinct from gold due to their shifted surface plasmon resonance. Multicolor, multiplexed lateral flow immunoassays can be performed with combinations of gold and gold-alloy nanoparticles.

40 nm Gold Nanoparticles

Size Optimized for Lateral Flow

Product Features:

No capping agents or surfactants
High-purity laser-fabrication
Bare surface for efficient conjugation
No capping agents or surfactants
High colloidal stability during conjugation
Long shelf-life
Red color
Size optimized for lateral flow immunoassays
Use with 40 nm gold-platinum nanoparticles for multiplex lateral flow

Specifications:

Average Size (by disc centrifugation): 40 ± 3 nm
Size Distribution (%CV): <18%
Peak Wavelength: 524.0 ± 2.5 nm

Protocols:

Passive Conjugation of Proteins to i-colloid 40 nm OD1 Gold and Gold-Platinum Nanoparticles
Passive Conjugation of Proteins to i-colloid 40 nm OD5 and OD10 Gold Nanoparticles
i-colloid 40 nm Gold and Gold-Platinum Conjugation Optimization
Sequential Conjugation to i-colloid 40 nm Gold Nanoparticles (coming soon)

Technical Notes:

Consistent Lateral Flow Performance with i-colloid 40 nm Gold
Equivalent Performance of i-colloid 40 nm Gold and Gold-Platinum
Multicolor, Multiplex Lateral Flow with i-colloid Nanoparticles

20 nm Gold Nanoparticles

Laser-fabricated, Surfactant-free

The 20 nm Gold nanoparticles are fabricated by pulsed-laser ablation.

Product Features:

No capping agents or surfactants
High biomolecule conjugation
High purity and colloidal stability

Specifications:

Average Size (by disc centrifugation): 20 ± 2 nm
Size Distribution (%CV): <25%
Peak Wavelength: 521.0 ± 2.5 nm

Protocols:

Passive Conjugation of Proteins to i-colloid 20 nm Gold
I-colloid Gold and Gold-Platinum Conjugation Optimization
Sequential Conjugation to i-colloid 20 nm Gold

Technical Notes:

Imaging with Multi-functionalized i-colloid 20 nm Gold

40 nm Gold-Platinum Nanoparticles

Laser-fabricated, surfactant-free

The 40 nm Gold-platinum nanoparticles are fabricated by pulse-laser ablation.

Product Features:

High biomolecule conjugation with no capping agents or surfactants
Homogeneous Gold and Platinum distribution
Black color from absorption across entire visible spectrum
High purity and colloidal stability
Size optimized for lateral flow immunoassays
Use with 40 nm gold nanoparticles for multiplex lateral flow

Specifications:

Average Size (by disc centrifugation): 40 ± 5 nm
Gold-Platinum absorbs in entire visible spectrum: OD is measured at 400 nm

Protocols:

Passive Conjugation of Proteins to i-colloid 40 nm Gold and Gold-Platinum
i-colloid Gold and Gold-Platinum Conjugation Optimization

Technical Notes:

Equivalent Performance of i-colloid 40 nm Gold and Gold-Platinum
Multicolor, Multiplex Lateral Flow with i-colloid Nanoparticles

Lateral Flow Immunoassay

Products for Lateral Flow